Wallin singled out Tory senators Marjory LeBreton and Carolyn Stewart Olsen on Wednesday as her main tormentors in what she described as an ongoing "show trial" and "charade" and demanded due process.
"You all now know I was subjected to a secret investigation, an extended audit process in which details of my life and activities were leaked to the media, my reputation was attacked and I was summarily thrown out of the Conservative party," she told her Senate colleagues.
Wallin is one of a trio of senators fighting allegations they abused red chamber resources and who are appealing to their peers to reject Conservative motions to suspend them without pay and benefits.
"Why is the Senate acting as accuser, judge, jury and executioner before I've had that day in court?" she asked.
Wallin - appointed by Prime Minister Stephen Harper, who has defended her in the past - also accused the Senate of a "double standard" in how questions about Stewart Olsen's own living expenses were handled behind closed doors.
Stewart Olsen, who resigned Wednesday from a secretive Senate committee that reviews upper chamber expenses, did not respond to a request for comment.
Senators Mike Duffy and Patrick Brazeau spoke in their own defence Tuesday.
LeBreton spent 15 minutes denying Wallin's accusations.
"The suggestion that I would ever, ever do anything to harm an individual, a human being, for any reason is appalling. I wouldn't do that to my worst enemy," she said. "It's false, false, false."
Following Wallin's statement, a number of her boosters spoke against the motions and argued senators had yet to review any of the documents in the affair, including external audits.
"We have absolutely no evidence upon which to base this extraordinarily important, severe decision," Liberal Sen. Joan Fraser said.
On Tuesday, Senate Opposition Leader James Cowan proposed an amendment to strike a special televised committee to allow the trio to plead their case.
Wallin has repaid more than $150,000 in expenses the Senate deemed inappropriate.
In an external audit, bookkeepers determined that 27% of Wallin's $532,508 in travel claims between 2009 and 2012 were for personal, business or partisan trips - not Senate business.
Wallin maintains the audit is deeply flawed, in part because her billings from 2009 on were retroactively judged under rules that came into effect three years later.
The Senate referred that audit to the RCMP.
Senators adjourned debate on the motions Wednesday night and will resume Thursday