Sun Media is cutting about 200 jobs across the chain due to "unprecedented changes in the print media industry driven largely by the digital revolution."
About a quarter of those jobs come from Sun Media's editorial departments.
"It is very tough to announce job cuts," said Julie Tremblay, president and CEO.
"But as distressing as they are for the employees involved, these restructuring initiatives are necessary to maintain our leading position and ensure the corporation's sustainability.
"We truly regret this decision's impact on the professionals who are leaving our organization. We thank each and every one of them and we wish them every success."
This follows 250 job cuts at Sun Media in July.
Job cuts have been common across Canadian media companies in recent years due to industry-wide print revenue losses.
Sun Media, a subsidiary of Quebecor Inc. and owner of QMI Agency, is Canada's largest newspaper publisher, with 39 daily newspapers and about 200 community papers.